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Diana Rhoads
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NewsAPRIL 3, 2007 Microscopy Laboratory CollaborationThe College of Engineering Materials Sciences Center and the College of Agricultural and Life Sciences Biological and Biomaterials Preparation, Imaging and Characterization Laboratory (BBPIC) announce a cooperative program to enhance the availability of state-of-the-art imaging and analytic technology for investigators in the biological and engineering sciences. The rapid development of collaborative studies involving biology and engineering has accentuated the need for cross discipline imaging which provides cutting edge preparative and imaging/analytic technology. Brief Laboratory Descriptions:The Materials Science Center:The Materials Science Center provides instrumentation and expertise for the characterization of materials and nanostructures. It houses three scanning electron microscopes (soon to be four) and 3 transmission electron microscopes, multiple x-ray diffractometers, two surface analysis instruments, and an atomic force microscope. Recent acquisitions include a dual-beam focused ion beam instrument, a scanning Confocal micro-Raman spectrometer, and a small-angle x-ray diffractometer. Four expert professional staff members train users and can help design experiments and interpret their results. The MSC is open on a recharge basis to member of the university and the broader community. Please see the MSC web site, msc.engr.wisc.edu, for more information. The Biological and Biomaterials Preparation, Imaging and Characterization Laboratory (BBPIC)The BBPIC was founded 20 years ago primarily as a facility to prepare biological and mixed biological-materials specimens for examination by light and electron microscopy. Development and application of advanced labeling technology for correlative light and electron microscopy of living and fixed specimens has also been a major BBPIC focus. Currently the BBPIC operates a conventional LaB6scanning electron microscope with secondary and backscattered electron detection capabilities. Also available is a high resolution field emission SEM with an in-lens detection system optimized for operation at low accelerating voltages. This instrument is also equipped with an Autrata high resolution backscattered electron detector and a cryostage for examination of frozen specimens. In addition the BBPIC maintains a Zeiss spinning disc confocal inverted light microscope with a high sensitivity Andor electron multiplying CCD camera. The spinning disc configuration and high sensitivity camera allows for real time imaging, use of a wide range of excitation and emission wavelengths for fluorescence microscopy, and live cell imaging. The BBPIC provides a wide range of ancillary technology for the preparation of biological and biomaterials specimens. Standard procedures include conventional fixation, critical point drying, and sputter coating. Instrumentation and expertise is available for specialized procedures including ultrarapid freezing, cryoetching and crycoating as well as cryofracture, freeze substitution, freeze drying, and ion beam sputter coating. Over the past 5 years nearly 80 different laboratories in 6 different colleges or schools on the UW Madison Campus have utilized facilities at the BBPIC. A number of individuals from private, government, and other university laboratories have also been assisted with specimen preparation and/or imaging at the BBPIC facility.
Contact Information:
Address
Manager:
Joe Heintz, Department of Animal Sciences
Director: Ralph M. Albrecht Department of Animal Sciences
Associate Director: Donna M. Peters, School of Medicine,
Department of Pathology and Laboratory Medicine
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Content: noll@engr.wisc.edu
Copyright 2007 The Board of Regents of the University of Wisconsin System |